hgf cells  (ATCC)

Journal: Scientific Reports

Article Title: Reactive oxygen species-sensitive release of cephalexin from hyaluronic acid- g -cephalexin dimer copolymer for treatment of inflammation and infection of oral soft tissue cells

doi: 10.1038/s41598-025-30912-7

Figure Lengend Snippet: Intrinsic cytotoxicity of CPX or HAgCPX nanoparticles. ( A ) HGF-1 cells; ( B ) L929 cells. CPX or HAgCPX nanoparticles in serum free media were treated to cells for 1 day. CPX in DMSO was diluted more than 100 times with cell culture media and then treated to cells. HAgCPX nanoparticles and/or free HA in aqueous solution were sterilized with a 1.2 µm syringe filter and then diluted cell culture media following treatment against cells. The significance is as follows: ns; not significant.

Article Snippet: Human gingival fibroblast-1 (HGF-1) cells were purchased from American Type Culture Collection (ATCC, Manassas, Virginia, USA).

Techniques: Cell Culture

Journal: Scientific Reports

Article Title: Reactive oxygen species-sensitive release of cephalexin from hyaluronic acid- g -cephalexin dimer copolymer for treatment of inflammation and infection of oral soft tissue cells

doi: 10.1038/s41598-025-30912-7

Figure Lengend Snippet: Anti-inflammatory effect of CPX or HAgCPX nanoparticles against LPS-treated HGF-1 cells. ( A ) Cell viability; ( B ) Expression of inflammatory cytokines such as IL-6 and TNF-α. For evaluation of inflammatory cytokines, CPX or HAgCPX nanoparticles were treated to cells. Final concentration was 20 µg/mL based on CPX) were treated to cells. HAgCPX nanoparticles in aqueous solution were diluted with media to be 20 µg CPX/mL. ( C ) Wound healing assay. Cells were treated with CPX or HAgCPX nanoparticles for 12 h. The dose of drug was 20 µg/mL based on CPX both of CPX or HAgCPX nanoparticles. After that, LPS (10 μg/ml) was treated to cells for 12 h. Cells were scratched and the cells were cultured for 48 h. The significance is as follows: #### p < 0.0001 compared with the control group; ** p < 0.01, *** p < 0.001, **** p < 0.0001 compared with the LPS control group; n., not significant.

Article Snippet: Human gingival fibroblast-1 (HGF-1) cells were purchased from American Type Culture Collection (ATCC, Manassas, Virginia, USA).

Techniques: Expressing, Concentration Assay, Wound Healing Assay, Cell Culture, Control

Journal: Scientific Reports

Article Title: Reactive oxygen species-sensitive release of cephalexin from hyaluronic acid- g -cephalexin dimer copolymer for treatment of inflammation and infection of oral soft tissue cells

doi: 10.1038/s41598-025-30912-7

Figure Lengend Snippet: Apoptosis/necrosis analysis of HGF-1 cells. Cells were treated with CPX or HAgCPX nanoparticles. The dose of drug was 20 µg/ml based on CPX. HAgCPX nanoparticles in aqueous solution were diluted with media to be 20 µg CPX/mL. These were washed with PBS once more and then LPS (10 μg/ml) was treated to cells for 12 h. FITC–annexin V (FITC-A) and propidium iodide (PI) were used to analyze apoptosis and necrosis, respectively.

Article Snippet: Human gingival fibroblast-1 (HGF-1) cells were purchased from American Type Culture Collection (ATCC, Manassas, Virginia, USA).

Techniques:

Journal: Scientific Reports

Article Title: Reactive oxygen species-sensitive release of cephalexin from hyaluronic acid- g -cephalexin dimer copolymer for treatment of inflammation and infection of oral soft tissue cells

doi: 10.1038/s41598-025-30912-7

Figure Lengend Snippet: Effect of LPS on the expression of CD44 receptor in the HGF-1 cells. ( A ) Expression of CD44 receptor in HGF-1 cells: (a) Fluorescence microscopy and (b) Flow cytometry. ( B ) CD44 receptor interaction of rhodamine B-labelled HAgCPX nanoparticles (HAgCPX NP) with LPS-treated HGF-1 cells: (a) Fluorescence microscopy; (b) flowcytometry. Free HA (0 mg/ml, 1 mg/ml or 10 mg/ml) was added to cell culture to block CD44 receptor of LPS-treated HGF-1 cells. The dose of drug was 20 µg/ml based on CPX. HAgCPX nanoparticles in aqueous solution were diluted with media to be 20 µg CPX/mL. One h later, cells were treated with rhodamine B-labelled HAgCPX nanoparticles for 90 min. Bar = 100 μm.

Article Snippet: Human gingival fibroblast-1 (HGF-1) cells were purchased from American Type Culture Collection (ATCC, Manassas, Virginia, USA).

Techniques: Expressing, Fluorescence, Microscopy, Flow Cytometry, Cell Culture, Blocking Assay

Journal: Scientific Reports

Article Title: Reactive oxygen species-sensitive release of cephalexin from hyaluronic acid- g -cephalexin dimer copolymer for treatment of inflammation and infection of oral soft tissue cells

doi: 10.1038/s41598-025-30912-7

Figure Lengend Snippet: Effect of pre-treatment of free HA on the secretion of cytokine. Secretion of inflammatory cytokines such as IL-6 ( A ) and TNF-α ( B ). CD44 receptor of HGF-1 cells was blocked with pre-treatment of free HA (final concentration, 1 mg/mL) for competition assay. For evaluation of inflammatory cytokines, CPX or HAgCPX nanoparticles were treated to cells. Final concentration was 20 µg/mL based on CPX) were treated to cells. HAgCPX nanoparticles in aqueous solution were diluted with media to be 20 µg CPX/mL. The significance is as follows: #### p < 0.0001 compared with the control group; **** p < 0.0001 compared with the LPS group; ns, not significant.

Article Snippet: Human gingival fibroblast-1 (HGF-1) cells were purchased from American Type Culture Collection (ATCC, Manassas, Virginia, USA).

Techniques: Concentration Assay, Competitive Binding Assay, Control